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KMID : 0380020010160060632
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Korean Journal of Biotechnology and Bioengineering
2001 Volume.16 No. 6 p.632 ~ p.637
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Biodegradation of Diesel Oil by Microorganisms Isolated from Petroleum Contaminated Site
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Park Chun-Bo
Hur Byung-Ki
Yun Hyun-Shik
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Abstract
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The cells obtained from diesel contaminated site were tested for diesel degradation by culturing them on the culture
medium that contained diesel as the only carbon source. Two strains that grew well in the culture media were
separated: one formed white colony and another strain formed yellow colony. When they were cultured together, much
higher diesel degradation was obtained compared to that of individual cell culture. Mixed culture of white and yellow
colony forming strains grew well with 1%(v/v) diesel and the addition of growth nutrients increased the diesel
degradation. Additional nitrogen source was efficient for higher diesel degradation (over 90%) when it was compared
with that without nitrogen source. When mixed culture of white and yellow colony forming cells were applied to the soil
column system contaminated by diesel, 30 mL/min of air flow rate was found to be sufficient to degrade diesel oil.
The diesel degradation did not increase noticeably at higher flow rate. The addition of nitrogen source resulted in the
increase in diesel degradability
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KEYWORD
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bioremediation, biodegradation, soil columns, diesel oil
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